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| Alfenas,Poliane F.; Braz,Antonio Sérgio K.; Torres,Leonardo B.; Santana,Enilton N.; Nascimento,Ana Verônica S. do; Carvalho,Murilo G. de; Otoni,Wagner C.; Zerbini,F. Murilo. |
| Sixteen transgenic yellow passionfruit (Passiflora spp.) plants (R0) were obtained which express a non-translatable transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, derived from the genome of a Brazilian isolate of Cowpea aphid-borne mosaic virus (CABMV). The transgenic plants were propagated by stem cuttings and challenged by sap inoculation with isolates CABMV-MG1 and CABMV-PE1. One transgenic plant (TE5-10) was resistant to the isolate CABMV-MG1, but susceptible to CABMV-PE1. The remaining transgenic plants developed systemic symptoms, equal to non-transformed plants, when inoculated with either isolate. The absence of virus in TE5-10 plants was confirmed by indirect ELISA. Transcription analysis of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passionfruit woodiness virus; PWV; Potyvirus; Resistance. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100006 |
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